duplex molecule of dna or of rna and dna
S14 A and C. In cleavage assays using plasmid DNA we observed that the longer chimeric RNA was able to guide Cas9-catalyzed DNA cleavage in a manner similar to that observed for the truncated tracrRNAcrRNA duplex Fig.
Thermodynamic Cycle For The Formation Of A Rna Dna Hybrid Green Blue Download Scientific Diagram
The shorter chimeric RNA did not work efficiently in this assay confirming that nucleotides that are 5 to 12 positions beyond.
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